BioChemical Techniques

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This is an industry oriented program to provide a thorough knowledge on the test methods and precautions to be taken for carrying out microbiological tests of various samples. Microbiology testing needs to be conducted with absolute accuracy to ensure hygiene and safety. We possess a well-equipped laboratory with all the modernized equipments and provide complete laboratory facilities and mentorship for students undergoing various graduates and undergraduates program in Microbiology, Biotechnology and allied subjects. The duration of the training program will be for 5days.

Topic Includes

• Introduction to Good Laboratory Practices
• Basics of Calculations; Buffers and Reagent Preparation
• Various Sterilization Techniques
• Culture Media (Solid/Liquid) preparation and its types
• Various methods for isolation of Cultures( Streak, Spread and pour plate techniques)
• Microbial Enumeration Techniques and CFU ( Colony Forming Unit) Count
• Antimicrobial Susceptibility test( Kirby-Bauer Method)
• MIC and MLC detection
• Isolation and Culturing of microbes from various samples (Soil, Water, Air)
• Purification of bacterial colonies from mixed population
• Identification of microorganisms by Biochemical methods
• Staining techniques

Beneficiaries

DMLT/ B.Sc. MLT/ Microbiology/ Biochemistry/Biotechnology Graduates & Post graduates)

Program Schedule

5 Days

• Day 1Introduction to Good Laboratory Practices• Basics of Calculations; Buffers and Reagent Preparation
• Day 2Various Sterilization Techniques• Culture Media (Solid/Liquid) preparation and its types• Various staining techniques
• Day 3Various methods for pure culture isolation ( Streak, Spread and pour plate techniques)• Microbial enumeration techniques and CFU ( Colony Forming Unit) count• Isolation and purification of microbes from various samples
• Day 4Water quality testing• Antimicrobial Susceptibility test( Kirby-Bauer Method)• MIC and MLC detection
• Day 5Microbiology testing of various food samples• Identification of microorganisms by Biochemical methods